Methods in microbiology Vol. 35 Extremophiles / edited by Fred A Rainey and Aharon Oren.

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This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of "standard" microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of "standard" microbiology protocols that have to be modified to be suitable for handling extremophiles.

Innehållsförteckning levererad av Syndetics

• Contributors (p. xi)
• 1 Extremophile Microorganisms and the Methods to Handle Them (p. 1)
• Thermophiles
• 2 In Situ Activity Studies in Thermal Environments (p. 29)
• 3 The Isolation of Thermophiles from Deep-sea Hydrothermal Environments (p. 55)
• 4 Growth of Hyperthermophilic Microorganisms for Physiological and Nutritional Studies (p. 93)
• 5 Toward the Large Scale Cultivation of Hyperthermophiles at High-Temperature and High-Pressure (p. 109)
• 6 Analysis of Lipids from Extremophilic Bacteria (p. 127)
• 7 Membranes of Thermophiles and Other Extremophiles (p. 161)
• 8 Characterization and Quantification of Compatible Solutes in (Hyper)thermophilic Microorganisms (p. 173)
• 9 Functional Genomics of the Thermo-Acidophilic Archaeon Sulfolobus solfataricus (p. 201)
• 10 Heat Shock Proteins in Hyperthermophiles (p. 233)
• 11 Deep-sea Thermococcales and their Genetic Elements: Plasmids and Viruses (p. 253)
• 12 Genetic Systems for Thermus (p. 279)
• 13 Gene Transfer Systems for Obligately Anaerobic Thermophilic Bacteria (p. 309)
• 14 Hyperthermophilic Virus-Host Systems: Detection and Isolation (p. 331)
• 15 Preservation of Thermophilic Microorganisms (p. 349)
• Psychrophiles
• 16 Handling of Psychrophilic Microorganisms (p. 371)
• 17 Proteins from Psychrophiles (p. 395)
• Alkaliphiles
• 18 Cultivation of Aerobic Alkaliphiles (p. 439)
• 19 Isolation, Cultivation and Characterization of Alkalithermophiles (p. 451)
• Acidophiles
• 20 The Isolation and Study of Acidophilic Microorganisms (p. 471)
• Halophiles
• 21 Characterization of Natural Communities of Halophilic Microorganisms (p. 513)
• 22 Cultivation of Haloarchaea (p. 535)
• 23 Extraction of Halophiles from Ancient Crystals (p. 553)
• 24 The Assessment of the Viability of Halophilic Microorganisms in Natural Communities (p. 569)
• 25 Characterization of Lipids of Halophilic Archaea (p. 585)
• 26 Characterization of Organic Compatible Solutes of Halotolerant and Halophilic Microorganisms (p. 615)
• 27 Genetic Systems for Halophilic Archaea (p. 649)
• 28 The Isolation and Study of Viruses of Halophilic Microorganisms (p. 681)
• 29 Detection, Quantification and Purification of Halocins: Peptide Antibiotics from Haloarchaeal Extremophiles (p. 703)
• 30 Storage of Halophilic Bacteria (p. 719)
• Barophiles
• 31 Handling of Piezophilic Microorganisms (p. 733)
• Radiation-Resistant Microorganisms
• 32 Measuring Survival in Microbial Populations Following Exposure to Ionizing Radiation (p. 745)
• Strict Anaerobes
• 33 The Study of Strictly Anaerobic Microorganisms (p. 757)
• Applications of Extremophiles
• 34 Applications of Extremophiles: The Industrial Screening of Extremophiles for Valuable Biomolecules (p. 785)
• Index (p. 815)