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Master 100 of the most commonly performed lab tests



A Doody's Core Title for 2024 & 2023!



Clinical Laboratory Methods covers the 100 tests most often done in clinical labs. The description of each method covers the basic concept of the assay; clinically important information; the expense of the test (based cost of the reagents, supplies, and labor); the complexity of the test; and whether each method is manual, semi-automated, or highly automated, and each method includes a flowchart. The author avoids getting bogged down in unimportant details, focusing on only what you need to know to master these important processes.



Features



* Each testing-method description includes a flowchart

* Practice tests enable you to quiz yourself on what you have learned

* The expense of each method is described as low, moderate, or high

Innehållsförteckning levererad av Syndetics

• Preface (p. ix)
• Acknowledgments (p. xi)
• Introduction: Concepts in Clinical Laboratory Testing (p. xiii)
• 1 Methods in Clinical Immunology (p. 2)
• Antinuclear antibody (ANA) testing
• Protein electrophoresis (PEP)
• Immunoflxation to identify monoclonal immunoglobulins
• Flow cytometry
• Cryoglobulin analysis
• 2 Methods in Clinical Microbiology (p. 12)
• Gram stain
• Microbiologic culture and organism identification
• Blood cultures
• Antimicrobial sensitivity tests
• Direct and indirect immunofluorescence for antigen detection
• 3 Methods in Clinical Hematology (p. 22)
• Counting of blood cells with automated white blood cell differential count
• Peripheral blood smear analysis
• Sickle cell screening assay
• Hemoglobin analysis
• Erythrocyte sedimentation rate
• 4 Methods in Clinical Coagulation (p. 32)
• The PT (Prothrombin Time) and PTT (Partial Thromboplastin Time) Assays
• PT and PTT mixing studies
• Coagulation factor assays
• Von Willebrand factor assays
• Platelet aggregation
• 5 Methods in Transfusion Medicine and Blood Banking (p. 42)
• ABO/Rh typing
• Blood component preparation
• Blood crossmatch
• Direct antiglobulin test (DAT)
• Indirect antiglobulin test (LAT)
• Apheresis
• Western blot
• 6 Methods in Clinical Chemistry and General Methods (p. 56)
• Electrolyte measurements: Sodium (Na), Potassium (K), and Chloride (CI)
• Assays measuring concentration of compounds by spectrophotometry
• Blood gas measurements
• Urinalysis
• Nephelometry
• Enzyme-linked immunosorbent assay (ELISA)
• Immunoassay with all reagents in solution
• Latex agglutination
• Chromatography for separation, identification, and quantitation of substances in biologic fluids
• Mass spectrometry for molecular identification
• Newborn screening by liquid chromatography/mass spectrometry (LC-MS)
• 7 Point-of-Care Testing Methods (p. 78)
• Point-of-care glucose testing
• Point-of-care immunoassay on test strip
• 8 Methods Involving Chromosomal Analysis and Molecular Genetics (p. 82)
• Chromosomal Analysis
• The Karyotype
• Fluorescence in situ Hybridization (FISH)
• Molecular Genetic Testing
• Clinical Testing Using Molecular Genetic Analysis
• Molecular Genetic Testing: PCR Methods
• Polymerase Chain Reaction (PCR)
• Detection of a Genomic Alteration Using PCR
• Reverse Transcription PCR
• Quantitative PCR
• Molecular Genetic Testing: Array/Hybridization Methods
• DNA Microarray
• Comparative Genomic Hybridization (CGH)
• Detection of a Genomic Alteration Using CGH
• Molecular Genetic Testing: Nucleic Acid
• Sequencing Methods
• DNA Library Preparation for Exome Sequencing: Hybrid Capture Methods
• DNA Library Preparation for Targeted Sequencing: Amplicon Generation Method
• Sanger Sequencing
• Next-Generation Sequencing Short Read: Reversible Terminator Sequencing Method
• Next-Generation Sequencing Short Read: Semiconductor Sequencing Method
• Next-Generation Sequencing Long Read: Single-Molecule Real-Time Sequencing Method
• Next-Generation Sequencing Long Read: Strand Sequencing Protein Nanopore Method
• Next-Generation Sequencing Single-Cell Transcriptomics: "10 × Genomics" Method
• Next-Generation Sequencing Synthetic Long Read: "TruSeq" Method
• Detection of a Genetic Alteration Using Next Generation Sequencing Method
• Digital Sequencing
• Self-Assessment Questions (p. 125)
• Self-Assessment Answers (p. 133)
• Further Reading (p. 137)
• Clinical Laboratory Reference Values (p. 139)
• Index (p. 157)